Michael G. Nichols, MS, PhD
Michael G. Nichols, MS, PhD

Michael G. Nichols, MS, PhD

Professor
Program Director,
College of Arts and Sciences

Academic Appointments

Department

  • Physics

Position

  • Professor

Secondary Appointment

  • Biomedical Sciences

Education

  • Harvey Mudd College,B.S. Physics,1990
  • University of Rochester,M.A. Physics,1992
  • University of Rochester,Ph.D.,1996

Publications and Presentations

Articles

  • Zholudeva, L. V., Ward, K. G., Nichols, M. G., Smith, H. J. Gentamicin differentially alters cellular metabolism of cochlear hair cells as revealed by NAD(P)H fluorescence lifetime imaging, Journal of Biomedical Optics, 20, Article ID 051032, 2015
  • Autofluorescence Lifetime Imaging, CRC Press- Taylor and Francis Group, 2014
  • Ward, Kristina G., Zholudeva, Lana V., Nichols, Michael G., Jensen Smith, Heather Gentamiacin Differentially Affects OHC and IHC Metabolism as Revealed by NADH Fluorescence Lifetime Imaging, Biophysical Journal, 104, 305a, 2013
  • Nichols, Michael G., Zholudeva, Lyandysha V., Lehnerz, Marcus J., Desa, Danielle E., Meyer, Christian T. Metabolic Profiling of Multicell Tumor Spheroids by NADH Fluorescence and Spatially-Resolved Oximetry, Biophysical Journal, 104, 304a, 2013
  • Holman, Nathan, Hauger, Eric, Nichols, Michael, Sidebottom, David, Haas, Eric One and Two Photon Fluorescence Correlation Spectroscopy on Proteins in Glucose Solutions, Biophysical Journal, 104, 346a-347a, 2013
  • Hallworth, Richard, Stark, Kelsey, Zholudeva, Lyandysha V., Currall, Benjamin B., Nichols, Michael G. The Conserved Tetrameric Subunit Stoichiometry of Slc26 Proteins, Microscopy and Microanalysis, 19, 799-807, 2013
  • Hallworth, Richard, Nichols, Michael G. Prestin in HEK cells is an obligate tetramer, Journal of Neurophysiology, 107, 5-11, 2012
  • Vergen, J., Hecht, C., Zholudeva, L. V., Marquardt, M. M., Hallworth, R., Nichols, M. G. Metabolic Imaging Using Two-Photon Excited NADH Intensity and Fluorescence Lifetime Imaging, Microscopy and Microanalysis, 18, 761-770, 2012
  • Subunit Counting as an Approach to Membrane Protein Stoichiometry, Microscopy and Microanalysis, 18, 771-780, 2012
  • Gentamicin rapidly inhibits mitochondrial metabolism in high-frequency cochlear outer hair cells, PLoS One, 7, e38471, 2012
  • Tonniges, J., Hansen, M., Duncan, J., Bassett, M. J., Fritzsch, B., Gray, B. D., Easwaran, A., Nichols, Michael G. Photo- and bio-physical characterization of novel violet and near-infrared lipophilic fluorophores for neuronal tracing, Journal of microscopy, 239, 117-134, 2010
  • Ekpenyong, Andrew E., Posey, Carolyn L., Chaput, Joy L., Burkart, Anya K., Marquardt, Meg M., Smith, Timothy J., Nichols, Michael G. Determination of cell elasticity through hybrid ray optics and continuum mechanics modeling of cell deformation in the optical stretcher, Applied Optics, 48, 6344-6354, 2009
  • Matei, V. A., Feng, F., Pauley, S., Beisel, K. W., Nichols, M. G., Fritzsch, B. Near-infrared laser illumination transforms the fluorescence absorbing X-Gal reaction product BCI into a transparent, yet brightly fluorescent substance, Brain Research Bulletin, 70, 33-43, 2006
  • Tiede, L. M., Nichols, M. G. Photobleaching of reduced nicotinamide adenine dinucleotide and the development of highly fluorescent lesions in rat basophilic leukemia cells during multiphoton microscopy, Photochemistry and Photobiology, 82, 656-664, 2006
  • Barth, E. E., Hallworth, R., Nichols, M. G. A comparison of the sensitivity of photodamage assays in rat basophilic leukemia cells., Photochem Photobiol, 81, 556-562, 2005

Presentations

  • The Role of Complex I in Mitochondrial Reactive Oxygen Species Formation in Cochlear Sensory and Supporting Cells During Aminoglycoside Exposure, 2015
  • The Role of Complex I in Mitochondrial Reactive Oxygen Species Formation in Cochlear Sensory and Supporting Cells During Aminoglycoside Exposure, 2015
  • Metabolic profiling of the skin to monitor the onset and progression of squamous cell carcinoma through time- and wavelength-resolved fluorescence lifetime imaging, 2015
  • Shining a light on cellular metabolism of skin cancer through time- and wavelength-resolved multiphoton microscopy, 2014
  • Label free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy, 2014
  • Evaluating mitochondrial superoxide formation in cochlear cells during ototoxic antibiotic exposure, 2014
  • Metabolic profiling of cochlear dysfunction via two-photon fluorescence lifetime microscopy of NADH, 2014
  • Mitochondrial respiration studies suggest a novel mechanism for Aminoglycoside-induced hearing loss, 2014
  • Label free imaging of the regenerating human periodontal ligament and gingival tissue using second harmonic generation confocal microscopy, 2014
  • Metabolic Profiling of Cultured Murine Cochleae via Fluorescence Lifetime Microscopy, 2014
  • Differences in Cochlear Sensory and Supporting Cell Mitochondrial Metabolism Bias Free Radical Production, 2014
  • Endogenous Differences in Cochlear Sensory and Supporting Cell Mitochondrial Metabolism Bias Free Radical Production during Ototoxin Exposure, 2014
  • Two-Photon Fluorescence Lifetime Imaging of Cochlear Dysfunction, 2014
  • Metabolic Profiling of Cultured Murine Cochleae via Fluorescence Lifetime Microscopy, 2013
  • Assessing the Impact of Antibiotics on Mitochondrial Respiration, 2013
  • Measuring Cellular Refractive Indicies with Digital Holographic Microscopy in the Near Infrared, 2013
  • Measuring Cellular Refractive Indicies with Digital Holographic Microscopy in the Near Infrared, 2013
  • Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy, 2013
  • Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy, 2013
  • The Single-Molecule Approach to Membrane Protein Stoichiometry, 2013
  • Advanced Methods in Microscopy, 2013
  • Metabolic Profiling of Cochlear Dysfunction by Fluorescence Lifetime Microscopy, 2013
  • Assessing cellular metabolism by NADH FLIM and intensity-based measurements of monolayers and multicell tumor spheroids, 2013
  • Design and construction of a spatially-resolved oximeter for metabolic profiling of multicell tumor spheroids, 2013
  • Ototoxic antibiotic-induced changes in cochlear metabolism generate cell-damaging free radicals, 2013
  • Spatially resolved profiling of multicell spheroids by NADH fluorescence lifetime imaging microscopy, 2013
  • Two-photon fluorescence correlation spectroscopy of proteins in glucose solutions, 2013
  • Design and construction of a spatially-resolved oximeter for metabolic profiling of multicell tumor spheroids, 2013
  • Biophysical imaging with multiphoton microscopy, 2013
  • The Conserved Tetrameric Subunit Stoichiometry of Slc26 Proteins, 2013
  • Gentamicin Differentially Affects OHC and IHC Metabolism as Revealed by NADH Fluorescence Lifetime Imaging, 2013
  • Metabolic profiling of multicell tumor spheroids by NADH fluorescence and spatially-resolved oximetry, 2013
  • One and Two Photon Fluorescence Correlation Spectroscopy on Proteins in Glucose Solutions, 2013
  • Metabolic Imaging using Two-Photon excited NADH Fluorescence Intensity and FLIM, 2012
  • Fluorescence correlation spectroscopy of proteins in sugar solutions, 2012
  • Intensity- and lifetime-based analyses of endogenous differences in NADH metabolism in cochlear hair cells, 2012
  • Two-photon fluorescence intensity and lifetime imaging of NADH reveals glucose availability in cultured cells, 2012
  • Fluorescence Correlation Spectroscopy of Tryptophan-containing Proteins in Sugar Solutions Using Two Photon Excitation, 2012
  • Prestin in Membranes Is a Tetramer, 2012
  • Two-photon fluorescence intensity and lifetime imaging microscopy of cultured cells, 2011

Research and Scholarship

Grant Funding Received

  • Metabolic Imaging of Disease progression in Skin Cancer by FLIM
  • NADH Fluorescence Lifetime-Based Analysis of Mitochondrial Disfunction during Ototoxic Antibiotic Exposure
  • Comparison of NADH FLIM and Intensity Imaging to Assess Cellular Energetics
  • Comparison of NADH FLIM and Intensity Imaging to Assess Cellular Energetics
  • Comparison of NADH FLIM and Intensity Imaging to Assess Cellular Energetics
  • Light Scattering and Fluorescence Study of Sugar Solutions as Cryoprotective Agents in Protein Storage