Josef (Jody) D. Franke, PhD
Josef (Jody) D. Franke, PhD

Josef (Jody) D. Franke, PhD

College of Arts and Sciences

Academic Appointments


  • Biology


  • Instructor

Publications and Presentations


  • Franke, JD. Approaches for using animal models to identify loci that genetically interact with human disease-causing point mutations. In Vitro Mutagenesis Protocols (3rd Edition) 2010, 634:203-213. PMID 20676986., Springer Publishing, 203-213, 2010


  • Erythrosin B: a versatile colorimetric and fluuorescent vital dye for bacteria. Josef D Franke, Ann L Braverman, Alison M Cunningham, Erin Eberhard and Greg Perry, Biotechniques, 68, 7 - 13, 2019
  • Sterol synthesis is essential for viability in Gemmata obscuriglobus
    Luke R. Gudde, Martin Hulce, Alexander H. Largen and Josef D. Franke
    FEMS Microbiology Letters
    Volume 366
    Issue 3, FEMS MIcrobiology Letters, N/A, N/A, 2018
  • Assembly of a complete genome sequence for Gemmata obscuriglobus reveals a novel prokaryotic rRNA operon gene architecture.
    Josef D. Franke, Wilson R. Blomberg, Robert T. Todd, Robert W. Thomas and Anna M. Selmecki, Antonie Van Leeuwenhoek, 111(11), 2095-2105, 2018
  • Development of a chemically-defined minimal medium for studies on growth and protein uptake of Gemmata obscuriglobus
    Henry P. Mishek, Stephen A. Stock, Jacqueline D.E Florick, Wilson R. Blomberg, Josef D. Franke, Journal of Microbiological Methods, 145, 40-46, 2017
  • Fluorogen activating protein-affibody probes: Modular, no-wash measurement of epidermal growth factor receptors. Wang Y, Telmer CT, Schmidt BF, Franke JD, Ort S, Arndt-Jovin DJ and Bruchez, MP Bioconjugate Chemistry. DOI: 10.1021/bc500525b, Bioconjugate Chemistry, 26 (1), 137-144, 2015
  • Atomic structure of the nuclear pore complex targeting domain of a Nup116 homologue from the yeast, Candida glabrata. Sampathkumar P, Kim SJ, Manglicmot D, Bain KT, Gilmore J, Gheyi T, Phillips J, Pieper U, Fernandez-Martinez J, Franke JD, Matsui T, Tsuruta H, Atwell S, Thompson DA, Emtage JS, Wasserman SR, Rout MP, Sali A, Sauder JM, Almo SC, Burley SK. Proteins. PMID: 22544723, Proteins, 80(8), 2110-2116, 2012
  • Structure-function mapping of a heptameric module in the nuclear pore complex. Fernandez-Martinez J, Phillips J, Sekedat MD, Diaz-Avalos R, Velazquez-Muriel J, Franke JD, Williams R, Stokes DL, Chait BT, Sali A, Rout MP. Journal of Cell Biology. PMID: 22331846, Journal of Cell Biology, 196 (4), 419-34, 2012
  • Structure of the C-terminal domain of Saccharomyces cerevisiae Nup133, a component of the nuclear pore complex. Sampathkumar P, Gheyi T, Miller SA, Bain KT, Dickey M, Bonanno JB, Kim SJ, Phillips J, Pieper U, Fernandez-Martinez J, Franke JD, Martel A, Tsuruta H, Atwell S, Thompson DA, Emtage JS, Wasserman SR, Rout MP, Sali A, Sauder JM, Burley SK. Proteins. PMID: 21365675, Proteins, 79 (5), 1672-2, 2011
  • A novel method for the production of in vivo-assembled, recombinant Escherichia coli RNA polymerase lacking the ? C-terminal domain. Twist KA, Husnain SI, Franke JD, Jain D, Campbell EA, Nickels BE, Thomas MS, Darst SA, Westblade LF. PMID: 21416542, Protein Science, 20(6), 986-995, 2011
  • Nonmuscle myosin II is required for cell proliferation, cell sheet adhesion and wing hair morphology during wing morphogenesis. Franke JD, Montague RA and Kiehart DP. PMID: 20599890., Developmental Biology, 345 (2), 117-32, 2010
  • Endocytosis-like protein uptake in the bacterium Gemmata obscuriglobus.
    Lonhienne TG, Sagulenko E, Webb RI, Lee KC, Franke J, Devos DP, Nouwens A, Carroll BJ, Fuerst JA. , Proc Natl Acad Sci U S A, 107(29), 12883-12888, 2010
  • Structures of the autoproteolytic domain from the Saccharomyces cerevisiae nuclear pore complex component, Nup145.  
    Parthasarathy Sampathkumar, Sinem A. Ozyurt, Johnny Do, Kevin T. Bain, Mark Dickey, Logan A. Rodgers, Tarun Gheyi, Andrej Sali, Seung Joong Kim, Jeremy Phillips, Ursula Pieper, Javier Fernandez-Martinez, Joseph D. Franke, Anne Martel,Hiro Tsuruta, Shane Atwell, Devon A. Thompson, J. Spencer Emtage, Stephen R. Wasserman, Michael P. Rout, J. Michael Sauder, and Stephen K. Burley, Proteins, 78(8), 1992-1998, 2010
  • The Compartmentalized Bacteria of the Planctomycetes-Verrucomicrobia-Chlamydiae Superphylum Have Membrane Coat-Like Proteins.
    Rachel Santarella-Mellwig, Josef Franke, Andreas Jaedicke, Matyas Gorjanacz, Ulrike Bauer, Aidan Budd, Iain W. Mattaj, Damien P. Devos, PLoS Biology, (8)1, 1-11, 2010
  • An MYH9 Human Disease Model in Flies: site-directed mutagenesis of the Drosophilanonmuscle myosin II results in hypomorphic alleles with dominant character.
    Franke JD, Montague RA, Rickoll  WL and Daniel P. Kiehart., Human Molecular Genetics, 16(24), 3160-3173, 2007
  • Native nonmuscle myosin II stability and light chain binding in Drosophila melanogaster
    Franke JD, Boury AL, Gerald NJ and Kiehart DP, Cell Motility and the Cytoskeleton, 63(10), 604-622, 2006
  • Nonmuscle Myosin II Generates Forces that Transmit Tension and Drive Contraction in Multiple Tissues during Dorsal Closure
    Franke JD, Montague RA, Kiehart DP. , Current Biology, 15(24), 2208-2201, 2005
  • Myosin VIIA Defects, which Underlie the Usher 1B Syndrome in Humans, Lead to Deafness in Drosophila. 
    Todi S, Franke JD, Kiehart DP, Eberl D, Current Biology, 15(9), 862-868, 2005
  • Rod mutations associated with MYH9-related disorders disrupt nonmuscle myosin-IIA assembly.
    Franke JD, Dong F, Rickoll WL, Kelley MJ, Kiehart DP, Blood, 105(1), 161-169, 2004
  • Drosophila crinkled, mutations of which disrupt morphogenesis and cause lethality, encodes fly myosin VIIA. 
    Kiehart DP, Franke JD, Chee MK, Montague RA, Chen TL, Roote J, Ashburner M. , Genetics, 168(3), 1337-1352, 2004
  • Actin dynamics: the arp2/3 complex branches out. (REVIEW)
    Kiehart DP and Franke, JD., Current Biology, 12(16), R557-559, 2002

Research and Scholarship

Grant Funding Received

  • UNO Subgrant Number: 2013-035. National Institutes of Health Award to Carnegie Mellon University 2U54RR022241-06 Subgrant Project Title: Continued development of intracellular fluoromodules Award Amount: $15,000. Award Duration: 09/15/2012 – 07/14/2013.
  • Studying the structure of the NPC core scaffold at high resolution F32 GM082029 (National Institutes of Health Postdoctoral Fellowship)
  • Characterizing the molecular mechanisms by which rod mutations in MYH6, MYH7 and MYH11 cause cardiac diseases. American Heart Association Postdoctoral Fellowship (10POST3160008)
  • Improved Separation of Carotenoids from Gemmata obscuriglobus CURAS Faculty Research Fund, Creighton University

Awards and Honors

  • American Heart Association Postdoctoral Fellowship, American Heart Association, 2010